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Casein Acid Hydrolysate which is free of vitamins can be used as a nitrogen source in culture media that is being used to conduct a bacteriological assay on vitamins and antibiotic sensitivity.
 
Casein Acid Hydrolysate is obtained by degrading casein with hydrochloric acid; vitamins and other growth-promoting compounds are largely destroyed in the process.
 
However, it is a rich source of amino acids and serves as a nitrogen source. It is suitable for the preparation of culture media employed in the bacteriological assay of vitamins and antibiotic sensitivity.